Application and Technical Notes
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Cultivation of Suspended Plant Cells in the BioLector
Monitoring cultivations of suspended plant cells is often a laborious task. Especially because very few suitable online monitoring techniques are available in microliter scale. This gap is closed by using BioLector technology. The screening of cell lines or media optimization can be carried out conveniently, even along with design of experiments (DoE). Up to now, the BioLector was mostly used for the fermentation of bacteria or yeasts. In this application note, it is asserted that with the example of Nicotiana tabacum, that the BioLector technology can be utilized for suspended plant cell cultivation in microliter scale.
Biomass Detection in Hydrolyzed Cellulosic Material
The fermentation of hydrolyzed cellulosic material presents multiple difficulties to researcher, including the use of substrate material with dark, opaque coloring. For this reason, the BioLector's ability to monitor biomass online is impeded. But with BioLector technology, it is not only possible to measure biomass via scattered light, but also by means of biogenic fluorescence of the cells, such as by riboflavin (GFP) or NAD(P)H signal. This application note shows the ability of the fluorescence signals measured by the BioLector to overcome a major hurdle in detection of biomass in hydrolysates from cellulosic feedstocks in 48 parallel cultivations.
The BioLector's Scattered Light Signal
With the BioLector technology, it is not only possible to monitor biomass concentration online in microtiter plates in the form of scattered light, but also noninvasively and in a parallel manner in 48 wells. Conventionally, biomass analysis is performed offline after taking a sample, which is laborious and naturally confined to certain time points. In this application note, the principle of the BioLector's operation will be explained, and the factors that may influence experimental results. Furthermore, correlation to traditional biomass measurements is demonstrated, including the procedure of integrating this calibration function in the BioLection software.
BioLection Analysis Software
By means of the BioLector technology, 48 experiments can be executed, monitored online, and displayed in parallel. The accompanying BioLection software is a user-friendly tool that can be used not only to define and supervise experiments, but also analyze measurement data as well. In this application note, all of the basic features of the BioLection software are described and an outline of the extensive application of the software is given.
Anaerobic High-Throughput Fermentations in the BioLector
Due to the volatility of the oil market, anaerobic bioprocesses are having a resurgence. Detailed process information on anaerobic fermentations is usually derived from standard stirred tank reactors, where an anaerobic atmosphere can be easily maintained and online monitoring data gained. Microtiter plates are already applied for the study of anaerobic organisms and processes in order to achieve high-throughput and lower cost and effort, but they lack the possibility of real-time measurement. In this application note, the adaption of the online monitoring BioLector technology to anaerobic requirements and its potential will be discussed.
RoboLector – Microbioreactor System for DO-Controlled Fed-Batch Cultivation
Bioprocesses ranging from bench to production scale are commonly operated in fed-batch mode. In contrast, up to now, performing fed-batch cultivations in microtiter plates has been difficult to realize. With the RoboLector system, it is now possible to perform up to 48 individually-controlled fed-batch cultivations at the same time and more. Based on all online recorded cultivation data within the BioLector, the liquid handling robot can carry out pre-defined manipulation actions, including sampling and the addition of liquids, like inducers or nutrients. This application note presents a detailed description of the computational control to realize DO-triggered fed-batch cultivations.
Mode of Operation of Optical Sensors for pH-Value and Dissolved Oxygen
The major objective for the use of microtiter plates in bioprocess development is how to gain significant process data for characterization and scale-up. The BioLector device enables customers to not only monitor biomass development in 48 parallel microbioreactors online, but the noninvasive and online detection of pH value and dissolved oxygen is also permitted via optical sensors (optodes). Optodes consist of special dyes that respond to environmental conditions. This technical note depicts the concept of the optodes' operational mode.
Guide for Cultivation in Microplates
For many compelling reasons, microplates have been used in laboratories for several decades. They are especially suitable if high throughput is needed. To use microplates as cultivation vessels, intense knowledge about the proper use is necessary, in order to be able to subsequently perform meaningful scale-ups. This guide provides an overview of the characteristics of different types of microplates available on the market.