Media Development Kit

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The media development kit performs fed-batch fermentations in reactors smaller than 0.25 L and nourishes cells on demand.

The fed-batch media kit is designed to release glucose from higher polysaccharides within the medium solution. The glucose release is controlled by enzymatic hydrolysis of the polysaccharides. By a simple variation of the enzyme concentration, the glucose release rate and therefore also the growth and protein expression can be diversified. Thus, experiments under a broad variety of growth rates and gen regulation are possible.

Advantages

  • Glucose fed-batch in small scale
  • No pumps necessary
  • High protein levels
  • More soluble protein
  • High biomass concentrations
    (e.g., up to OD600 50 for E. coli, up to OD600 100 for P. pastoris)
  • Controlled linear growth
  • Controlled protein expression
  • No overflow metabolism
  • Better pH maintenance
  • Reduced oxygen requirements
  • Better cell viability

The fed-batch media kits are developed for the most common expression systems and optimized for work with BioLector microbioreactor technology. They provide high protein and biomass titers.

 

 

Select a media development kit

For Glucose Fed-Batch

M-KIT-100

  • 1 x 100 mL concentrated polysaccharide (100 g / L, liquid, sterile; function: glucose reservoir)
  • 1 x 10 mL concentrated enzyme (concentration: see label, liquid, sterile; function: hydrolysis of polysaccharide) for up to 300 mL media

M-KIT-500 

  • 1 x 500 mL concentrated polysaccharide (100 g / L, liquid, sterile; function: glucose reservoir)
  • 1 x 20 mL concentrated enzyme (concentration: see label, liquid, sterile; function: hydrolysis of polysaccharide) for up to 1.5 L media.
  • Media recipes for synthetic and complex media for E. coli, H. polymorpha, P. pastoris, S. cer.

 

These kits provide the sole source of carbon for your experiment; you must add your own culture media in order to grow cells.