BioLector

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Microbioreactor for high-throughput fermentations with the online monitoring of biomass, pH, DO, and fluorescences.

The BioLector I measures the most common fermentation parameters online while running a cultivation. Additionally, the bench top microbioreactor controls the shaking speed, the temperature inside the cultivation chamber, and the humidity.

///   BioLector® is a registered trademark.

Product Info

Real time culture monitoring on a parallel scale

 

Working Principle

The micro fermentation system is based on standard disposable microtiter plate formats and operates with non-invasive, optical sensors. Due to the continuous and rigorous shaking during the optical measurements and during the entire experiment, it is ideally suited for aerobic and anaerobic cultures.

Optional Modules

If a high or very low oxygen level is required, the system can be easily upgraded with additional modules, for example for CO2-enriched aeration, or anaerobic conditions.

By adding one of these modules the microbioreactor maintains all existing basic features. An upgrade with multiple modules in one system is possible.

///  Gas Modules

  • O2 up regulating module for the up-regulation of oxygen up to 35 %
  • O2 down regulating module for the down-regulation of oxygen down to 2 %
  • CO2 up regulating module for fermentation with a CO2-controlled gas atmosphere
  • Anaerobic cultivation set for cultivating under anaerobic conditions

///  Fluorescence Modules

  • LED / filter module for fluorescence measurements for wavelengths between 365 nm - 800 nm.

Up to three additional fluorescence modules can be added; in total, six different ones fit into the system.

 

Automated Fermentation

For fully-automated high-throughput cultivations, the BioLector will be combined with a liquid handling robotic system. Learn more about the RoboLector

Applications
  • Cell line and strain screening
  • Media screening and optimization
  • Fermentation parameter optimization
  • Anaerobic and microaerophilic fermentations
  • Synthetic and systems biology
  • Statistical design of experiments (DoE)
  • Growth characterization
  • Protein-building kinetics
  • High-throughput protein expression
  • Enzyme and cell activity tests
  • Functional genomics
  • Proteomic studies
  • Inhibition and toxicity tests
  • Quality control 
Advantages
  • Real-time kinetics of 48 parallel fermentations
  • Microfermentation in standard MTP format
  • High-throughput and easy automation
  • Broad range for biomass detection (up to 100 g/L CDW, 650 OD600)
  • No edge effects
  • Continuous shaking operation (no artifacts)
  • Defined mass transfer conditions (no Olimitation)
  • Easy scale up to lab-fermenters
  • Industry leading data analysis software
  • Fast and easy data analysis included
  • A valuable tool for PAT and QbD 
Online Parameters
  • Biomass concentration
  • pH value
  • Dissolved oxygen (DO)
  • NAD(P)H and riboflavins
  • Fluorescent molecules (GFP, YFP, DsRed, ...)
  • Temperature control (5 °C below room temperature) with active cooling
  • Humidity control (> 75 % rH)
  • Controlled gas atmosphere (CO2, O2, and N2)
Cultured Cells in the BioLector (extract)

Microorganisms

  • Bacillus subtilis
  • Candida spec.
  • Clostridium diolis and acetobutylicum
  • Corynebacterium glutamicum
  • Escherichia coli
  • Hansenula polymorpha
  • Lactobacillus spec.
  • Paracoccus spec.
  • Penicillium spec.
  • Pichia pastoris
  • Photorhabtus luminescens
  • Saccharomyces cerevisiae
  • Schizosaccharomyces pombe
  • Streptomyces spec.
  • Tetrahymena spec.
  • Vibrio natregiens spec.
  • Yarrowia lipolytica
  • Zymomonas spec.

 Plant cells

  • Nicotiana tabacum

Cell cultures

  • CAP
  • PerC6
  • Sf9 and Sf21