Call-backCulture media
Feed-In-Time (FIT) Fed-batch Media
Most of the industrial biotechnology processes are conducted in fedbatch mode due to the reduction of overflow metabolism of the expression host and the general higher productivity of this controlled process. But why is everybody still screening for production strains under batch conditions? The answer is easy. Up to know, there was no solution to perform fedbatch fermentations in reactors smaller than 0.25 L and on the other hand high producers will only be found in screening of hundreds or thousands of different clones. With the new FIT fedbatch media this bootleneck is released now. The FIT fedbatch media are designed to release glucose from higher polysaccharides within the medium solution. The glucose release is controlled by enzymatic hydrolysis of the polysaccharides. By a simple variation of the enzyme concentration the glucose release rate and therefore also the growth and protein expression can be diversified. Thus, experiments under a broad variety of growth rates and gen regulation is now possible. Apply Feed-In-Time fedbatch media and get cells and protein just in time when you need them!
Advantages of FIT fedbatch media:
Glucose fedbatch in small scale
No pumps necessary
High protein levels
More soluble protein
High biomass concentrations (OD 40-50)
Controlled linear growth
Controlled protein expression
No overflow metabolism
Better pH maintainance
Reduced oxygen requirements
Better cell viability
We have developed FIT fedbatch media for the most common expression systems such as Escherichia coli and Pichia pastoris. The media are optimized with our BioLector technology and provide high protein and biomass titer. Please choose your medium from the list below.
We are continuously developing new media for new expressions system.
Please do not hesitate to contact us and ask about media for your expression system.
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Fed-batch media for B. subtilis (synthetic) 1000ml
Product No. : M-Bs.syn-1000
Package Size : 2 Units
Package Price : 149 €Glucose fed-batch fermentation medium kit (synthetic) for Bacillus subtilis cultures, sterile, 1000ml (2 x 500ml)
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Fed-batch media for B. subtilis (synthetic) 125ml
Product No. : M-Bs.syn-125
Package Size : 1 Unit
Package Price : 29 €Glucose fed-batch fermentation medium kit (synthetic) for Bacillus subtilis cultures, sterile, 125ml
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Fed-batch media for B. subtilis (synthetic) 500ml
Product No. : M-Bs.syn-500
Package Size : 4 Units
Package Price : 99 €Glucose fed-batch fermentation medium kit (synthetic) for Bacillus subtilis cultures, sterile, 500ml (4 x 125ml)
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Fed-batch media for E. coli (complex) 1000ml
Product No. : M-Ec.com-1000
Package Size : 2 Units
Package Price : 149 €Glucose fed-batch fermentation medium kit (complex) for Escherichia coli cultures, sterile, 1000ml (2 x 500ml)
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Fed-batch media for E. coli (complex) 125ml
Product No. : M-Ec.com-125
Package Size : 1 Unit
Package Price : 29 €Glucose fed-batch fermentation medium kit (complex) for Escherichia coli cultures, sterile, 125ml
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Fed-batch media for E. coli (complex) 500ml
Product No. : M-Ec.com-500
Package Size : 4 Units
Package Price : 99 €Glucose fed-batch fermentation medium kit (complex) for Escherichia coli cultures, sterile, 500ml (4 x 125ml)
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Fed-batch media for E. coli (synthetic) 1000ml
Product No. : M-Ec.syn-1000
Package Size : 2 Units
Package Price : 149 €Glucose fed-batch fermentation medium kit (synthetic) for Escherichia coli cultures, sterile, 1000ml (2 x 500ml)
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Fed-batch media for E. coli (synthetic) 125ml
Product No. : M-Ec.syn-125
Package Size : 1 Unit
Package Price : 29 €Glucose fed-batch fermentation medium kit (synthetic) for Escherichia coli cultures, sterile, 125ml
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Fed-batch media for E. coli (synthetic) 500ml
Product No. : M-Ec.syn-500
Package Size : 4 Units
Package Price : 99 €Glucose fed-batch fermentation medium kit (synthetic) for Escherichia coli cultures, sterile, 500ml (4 x 125ml)
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Fed-batch media for H. polymorpha (synthetic) 1000ml
Product No. : M-Hp.syn-1000
Package Size : 2 Units
Package Price : 149 €Glucose fed-batch fermentation medium kit (synthetic) for Hansenula polymorpha cultures, sterile, 1000ml (2 x 500ml)
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Fed-batch media for H. polymorpha (synthetic) 125ml
Product No. : M-Hp.syn-125
Package Size : 1 Unit
Package Price : 29 €Glucose fed-batch fermentation medium kit (synthetic) for Hansenula polymorpha cultures, sterile, 125ml
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Fed-batch media for H. polymorpha (synthetic) 500ml
Product No. : M-Hp.syn-500
Package Size : 4 Units
Package Price : 99 €Glucose fed-batch fermentation medium kit (synthetic) for Hansenula polymorpha cultures, sterile, 500ml (4 x 125ml)
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Fed-batch media for P. pastoris (synthetic) 1000ml
Product No. : M-Pp.syn-1000
Package Size : 2 Units
Package Price : 149 €Glucose fed-batch Glucose-fedbatch fermentation medium kit (synthetic) for Pichia pastoris cultures, sterile, 1000ml (2 x 500ml)
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Fed-batch media for P. pastoris (synthetic) 125ml
Product No. : M-Pp.syn-125
Package Size : 1 Unit
Package Price : 29 €Glucose fed-batch fermentation medium kit (synthetic) for Pichia pastoris cultures, sterile, 125ml
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Fed-batch media for P. pastoris (synthetic) 500ml
Product No. : M-Pp.syn-500
Package Size : 4 Units
Package Price : 99 €Glucose fed-batch fermentation medium kit (synthetic) for Pichia pastoris cultures, sterile, 500ml (4 x 125ml)