2009 Publications

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Kensy F., Engelbrecht C. and Büchs J.
Scale-up from microtiter plate to laboratory fermenter: Evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations
E. coli and H. polymorpha were fermented in parallel at microtiter plate scale and stirred tank fermenter scale (1.4 L). The bioprocess kinetics were evaluated by optical online measurements of biomass and protein concentrations.
Microbial Cell Factories 2009, 8: 68, doi:10.1186/1475-2859-8-68
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Kottmeier K., Weber J., Müller M., Bley T. and Büchs J.
Asymmetric division of Hansenula polymorpha reflected by a drop of light scatter intensity measured in batch microtiter plates cultivations at phosphate limitation
Hansenula polymorpha RB11 pC10-FMD (PFMD− GFP) (FMD promoter gfp gene) was simultaneously cultivated in the Respiration Activity Monitoring System (RAMOS) and in the microtiter plate cultivation system “BioLector” under phosphate limitation.
Biotechnol. Bioeng., 104: 554–561. doi:10.1002/bit.22410
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Huber R., Ritter D., Hering T., Hillmer A.-K., Kensy F., Müller C., Wang L. and Büchs J.
Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content
The Robo-Lector generates extensive kinetic data in high-throughput cultivations, particularly for biomass and fluorescence protein formation. Based on the non-invasive on-line-monitoring signals, actions of the liquid-handling robot can easily be triggered.
Microbial Cell Factories 2009, 8: 42, doi:10.1186/1475-2859-8-42
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Kensy F., Zang E., Faulhammer F., Tan R.-K. and Büchs J.
Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates
Bacterial and yeast biomass concentrations of up to 50 g/L cell dry weight could be linearly correlated to scattered light intensities. In media screening, the BioLector demonstrated its potential for detecting different biomass and product yields, and...
Microbial Cell Factories 2009, 8: 31, doi:10.1186/1475-2859-8-31
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Funke M., Diederichs S., Kensy F., Müller C. and Büchs J.
The baffled microtiter plate: Increased oxygen transfer and improved online monitoring in small scale fermentations
The focus of this study is to investigate how baffling and the well geometry affect the maximum oxygen transfer capacity (OTRmax) in microtiter plates. On a 48-well plate scale, 30 different cross-section geometries of a well were studied.
Biotechnology & Bioengineering 2009, 103(6): 1118-1128, doi:10.1002/bit.22341
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Kensy F., Zimmermann H.F., Knabben I., Anderlei T., Trauthwein H., Dingerdissen U. and Büchs J.
Oxygen transfer phenomena in 48-well microtiter plates: Determination by optical monitoring of sulfite oxidation and verification by real-time measurement during microbial growth
A proportionality constant between the OTRmax of the biological system and the OTRmax of the chemical system were indicated from the data, offering the possibility to transform the whole set of chemical data to biologically relevant conditions.
Biotechnol. Bioeng., 89: 698–708. doi:10.1002/bit.20373
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