2010 Publications

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Funke M., Buchenauer A., Mokwa W., Kluge S., Hein L., Müller C., Kensy F. and Büchs J.
Bioprocess control in Microscale: Scalable fermentations in disposable and user-friendly microfluidic systems
A novel microfermentation system , the so-called 'microfluidic BioLector', has been presented. Microfluidic chips were coupled to MTPs to realize pH-controlled batch as well as fed-batch fermentations.
Microbial Cell Factories 2010, 9: 86, doi:10.1186/1475-2859-9-86
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Funke M., Buchenauer A., Schnakenberg U., Mokwa W., Diederichs S., Mertens A., Müller C., Kensy F. and Büchs J.
Microfluidic BioLector – Microfluidic Bioprocess Control in Microtiter Plates
The BioLector technology in comination with microfluidic control was described. Illustrations of Applications in fermentations of E.coli under pH-controlled and fed-batch conditions in shaken MTPs.
Biotechnology & Bioengineering 2010, 107(3): 497-505, doi:10.1002/bit.22825
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Drepper T., Huber R. Heck A., Circolone F., Hillmer A.-K., Büchs J. and Jaeger K.-E.
Flavin Mononucleotide-Based Fluorescent Reporter Proteins Outperform Green Fluorescent Protein-Like Proteins as Quantitative In Vivo Real-Time Reporters
The fluorescence signal intensity of GFP-like proteins is affected under oxygen limitation and therefore does not reflect the amount of reporter protein in Escherichia coli batch cultures.
Applied and Environmental Microbiology 2010, 76(17): 5990-5994, doi:10.1128/AEM.00701-10
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Huber R., Palmen T.G., Ryk N., Hillmer A.-K., Luft K., Kensy F. and Büchs J.
Replication methods and tools in highthroughput cultivation processes – recognizing potential variations of growth and product formation by on-line monitoring
The effects of replication methods and tools on growth kinetics of E.coli SCS1 pQE-30 pSE111 were observed and the influence on product formation was investigated. The Growth was monitored with the BioLector.
BMC Biotechnology 2010, 10: 22, doi:10.1186/1472-6750-10-22
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Scheidle M., Jeude M., Dittrich B., Denter S., Kensy F., Suckow M., Klee D. and Büchs J.
High-throughput screening of Hansenula polymorpha clones in the batch compared with the controlled-release fed-batch mode on a small scale
To show differences in ranking during strain selection depending on the operational mode, screenings were performed in batch and fed-batch modes. Two populations of Hansenula polymorpha were applied.
FEMS Yeast Research 2010, 10: 83-92, doi:10.1111/j.1567-1364.2009.00586.x
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Wulfhorst H., Jäger G., Ellinidou E., Büchs J. and Spiess A.
Charakterisierung von Cellulasepräparationen mittels Streulicht
Kinetic parameters and the pH optimum of a commercial cellulase preparation were determined, thus demonstrating the suitability of the BioLector technique for high-throughput characterization.
Chemie Ingenieur Technik 2010, 82(1-2) :1-5, doi:10.1002/cite.200900126
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