2014 Publications

2017 | 2016 | 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2009  

Gorochowski T. E., van den Berg E., Kerkman R., Roubos J. A. and Bovenberg R. A. L.
Using Synthetic Biological Parts and Microbioreactors to Explore the Protein Expression Characteristics of Escherichia coli
Creation of a combinatorial library of constructs that modulated expression strength of a GFP. A detailed large-scale assessment of transient expression and growth characteristics across several temperatures was performed.
ACS Synthetic Biology, 2014, 3(3): 129–139, doi:10.1021/sb4001245
Go to publication
Feng Y., De Franceschi G., Kahraman A., Soste M., Melnik A., Boersema P. J., Polverino de Laureto P., Nikolaev Y., Oliveira A. P. and Picotti P.
Global analysis of protein structural changes in complex proteomes
A method that couples limited proteolysis with a targeted proteomics workflow enables large-scale analyses of protein conformational changes directly in their biological matrices.
Nature Biotechnology, 2014, 32: 1036–1044, doi:10.1038/nbt.2999
Go to publication
Hemmerich J. and Kensy F.
Automated microbioreactor systems for pharmaceutical bioprocessing: profiling of seeding and induction conditions in highthroughput fermentations
Automated microbioreactor systems for intensive bioprocess characterization for conduction of precise inoculation procedures and for detailed studies on influential bioprocess Parameters.
Pharmaceutical Bioprocessing, 2014, 2(3): 227–235, doi:10.4155/pbp.14.18
Go to publication
Unthan S., Baumgart M., Radek A., Herbst M., Siebert D., Brühl N., Bartsch A., Bott M., Wiechert W., Marin K., Hans S., Krämer R., Seibold G., Frunzke J., Kalinowski J., Rückert C., Wendisch V. F. and Noack S.
Chassis organism from Corynebacterium glutamicum – a top-down approach to identify and delete irrelevant gene clusters
A top-down construction of a chassis organism from Corynebacterium glutamicum ATCC 13032 was initiated. Five combinatory deletions of irrelevant gene clusters were investigated.
Biotechnology Journal, 2015, 10(2): 290–301, doi:10.1002/biot.201400041 (2014)
Go to publication
Käß F., Prasad A., Tillack J., Moch M., Giese H., Büchs J., Wiechert W. and Oldiges M.
Rapid assessment of oxygen transfer impact for Corynebacterium glutamicum
A new procedure for oxygen transfer screening, assessing the impact of maximum oxygen transfer conditions within microtiter plate-based cultivation for enhanced throughput.
Bioprocess and Biosystems Engineering, December 2014, 37(12): 2567-2577, doi:10.1007/s00449-014-1234-1
Go to publication
Hemmerich J., Adelantado N., Barrigón J.M., Ponte X., Hörmann A., Ferrer P., Kensy F. and Valero F.
Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae Lipase
The performance of two fed-batch strategies for lipase production between the RoboLector microbioreactor and 5 L stirred tank bioreactor are compared. The scale-up from microbioreactor to 5 L bioreactor showed an excellent correlation.
Microbial Cell Factories, 2014, 13: 36, doi:10.1186/1475-2859-13-36
Go to publication
Siedler S., Schendzielorz G., Binder S., Eggeling L., Bringer S. and Bott M.
SoxR as a Single-Cell Biosensor for NADPH-Consuming Enzymes in Escherichia coli
Establishment of an ultra-high-throughput screening system for NADPH-dependent enzymes. Screening of a mutant LbAdh library for variants showing improved activity with the substrate 4-methyl-2-pentanone.
ACS Synthetic Biology, 2014, 3 (1): 41–47, doi:10.1021/sb400110j
Go to publication
Schendzielorz G., Dippong M., Grünberger A., Kohlheyer D., Yoshida A., Binder S., Nishiyama C., Nishiyama M., Bott M. and Eggeling L.
Taking Control over Control: Use of Product Sensing in Single Cells to Remove Flux Control at Key Enzymes in Biosynthesis Pathways
A plasmid-encoded ArgB of C. glutamicum was randomly mutagenized and the library was screened in a strain carrying the sensor pSenLys-Spc.
ACS Synthetic Biology, 2014, 3 (1): 21-29, doi:10.1021/sb400059y
Go to publication
Unthan S., Grünberger A., van Ooyen J., Gätgens J., Heinrich J., Paczia N., Wiechert W., Kohlheyer D. and Noack S.
Beyond Growth Rate 0.6: What Drives Corynebacterium glutamicum to Higher Growth Rates in Defined Medium
The general applicability of microfluidic single-cell cultivations to investigate and validate the effect of single medium components on microorganism growth during cultivation in liquid media.
Biotechnology and Bioengineering, 2014, 111 (2): 359–371, doi:10.1002/bit.25103
Go to publication
Mustafi N., Grünberger A., Mahr R., Helfrich S., Nöh K., Blombach B., Kohlheyer D. and Frunzke J.
Application of a Genetically Encoded Biosensor for Live Cell Imaging of L-Valine Production in Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum Strains
Application of Lrp-biosensor for monitoring of amino acid production in single cells of L-valine producing C. glutamicum strains.
PLoS ONE, 2014, 9 (1): e85731, doi:10.1371/journal.pone.0085731
Go to publication
Pereira F.
EuroSciCon meeting on bioprocess miniaturization: development and optimization26 November 2013, London, UK
The meeting reviewed individual operations in the bioprocess workflow highlighting the state-of-the-art in technology and research and innovation.
Pharmaceutical BioProcessing, 2014, 2 (2): 115–116, doi:10.2217/PBP.14.9
Go to publication
Nanda A. M., Heyer A., Krämer C., Grünberger A., Kohlheyer D. and Frunzke J.
Analysis of SOS-Induced Spontaneous Prophage Induction in Corynebacterium glutamicum at the Single-Cell Level
A PrecA-eyfp promoter fusion revealed that a small fraction of C. glutamicum cells exhibited a spontaneous induction of the SOS response.
Journal of Bacteriology, January 2014, 196 (1): 180-188, doi:10.1128/JB.01018-13
Go to publication