2016 Publications

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Freier L., Hemmerich J., Schöler K., Wiechert W., Oldiges M., von Lieres E.
Framework for Kriging-based iterative experimental analysis and design: Optimization of secretory protein production in Corynebacterium glutamicum
Merge of three different aspects: (1) Current technology of microbioreactor systems, (2) lab automation, and (3) Kriging-based experimental analysis and design. The goal: maximization of the product titer of secreted GFP, synthesized by C. glutamicum, through variation of media component concentrations.
Eng. Life Sci. 2016, 0, 1–12; doi: 10.1002/elsc.201500171
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Ladner T., Held M., Flitsch D., Beckers M. and Büchs J.
Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate
Online monitoring of Escherichia coli and Hansenula polymorpha cultures: the device simplifies the experimental efforts, instead of parallel cultures in a shake flask and MTP, just one single experiment in MTP needs to be conducted to measure the OTR, dissolved oxygen tension (DOT), scattered light and fluorescence.
Microbial Cell Factories 2016, 15:206, DOI: 10.1186/s12934-016-0608-2
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Jacques P., Béchet M., Bigan M. et al.
High-throughput strategies for the discovery and engineering of enzymes for biocatalysis
Novel enzyme discoveries for biocatalysis and biotransformations. Microfluidics/microplates, parallel miniscale bioreactors and others enable high-throughput screening methods and experimental design tools for screening and optimization, for the discovery, development and implementation of enzymes and whole cells in (bio)processes.
Bioprocess Biosyst Eng (2016), doi:10.1007/s00449-016-1690-x
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Back A., Rossignol T., Krier F., Nicaud J.-M. and Dhulster P.
High-throughput fermentation screening for the yeast Yarrowia lipolytica with real-time monitoring of biomass and lipid production
Small cultivation systems of the model yeast Yarrowia lipolytica have been developed that mimic the conditions in benchtop bioreactors. In an microbioreactor system, a BioLector, high-throughput fermentation procedures were set up that optimize growth and lipid accumulation in Y. lipolytica.
Microbial Cell Factories 2016, 15:147, doi: 10.1186/s12934-016-0546-z
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Motta Dos Santos L. F., Coutte F., Ravallec R., Dhulster P., Tournier-Couturier L., Jacques P.
An improvement of surfactin production by B. subtilis BBG131 using design of experiments in microbioreactors and continuous process in bubbleless membrane bioreactor
Culture medium elements were analysed by a screening DoE to identify their influence in surfactin specific production by a surfactin constitutive overproducing Bacillus subtilis strain.
Bioresource Technology 218 (2016) 944–952
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Neves D., Nies S., Ebert B. E., Blank L. M.
Comprehensive analysis and cross-species comparison of synthetic promoters
Escherichia coli and Pseudomonas taiwanensis VLB120 were chosen as test case for the development of promoters for standardized gene expression. σ70 promoter libraries expressing msfGFP were created and chromosomally integrated into thesingle attTn7 site in both organisms.
Poster at SBFC 2016 (38th Symposium on Biotechnology for Fuels and Chemicals), April 25-28, Baltimore, MD, USA
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Blesken C., Olfers T., Grimm A. and Frische N.
The microfluidic bioreactor for a new era of bioprocess development
Microfluidic components in a microbioreactor system achieve continuous feeding and pH control on an MTP Format with 32 bioreactor wells and 16 reservoir wells. 32 fed-batch cultivations can be run in parallel, completely automated, with extensive data output.
Engineering in Life Sciences, March 2016, 16(2): 190-193, doi: 10.1002/elsc.201500026
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Bleckmann M, Schürig M, Chen F-F, Yen Z-Z, Lindemann N, Meyer S, et al.
Identification of Essential Genetic Baculoviral Elements for Recombinant Protein Expression by Transactivation in Sf21 Insect Cells
Several vectors comprising different viral promoters or promoter combinations and tested them for eGFP expression using the automated BioLector microcultivation system were designed.
PLoS ONE, March 2016, 11(3): e0149424. doi:10.1371/journal.pone.0149424
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Bleckmann, M., Schmelz, S., Schinkowski, C., Scrima, A. and van den Heuvel, J.
Fast plasmid based protein expression analysis in insect cells using an automated SplitGFP screen
A high-throughput initial expression screening method is described using an automated microcultivation system in conjunction with fast plasmid based transient transfection in insect cells for the efficient generation of protein constructs.
Biotechnology and Bioengineering, March 2016, doi:10.1002/bit.25956
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