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Microbioreactor for high-throughput fermentations with the online monitoring of biomass, pH, DO, and fluorescences.

The BioLector II measures the most common fermentation parameters online while running a cultivation. Additionally, the bench top microbioreactor controls the shaking speed, the temperature inside the cultivation chamber, and the humidity.

With this microbioreactor we provide a solution for rapid response, COVID-19 vaccine development. → Read the testimonial here

///   BioLector® is a registered trademark.

Product Info

Real time culture monitoring on a parallel scale


Working Principle

The micro fermentation system is based on standard disposable microtiter plate formats and operates with non-invasive, optical sensors. Due to the continuous and rigorous shaking during the optical measurements and during the entire experiment, it is ideally suited for aerobic and anaerobic cultures.

Optional Modules

If a high or very low oxygen level is required, the system can be easily upgraded with additional modules, for example for CO2-enriched aeration, or anaerobic conditions.

By adding one of these modules the microbioreactor maintains all existing basic features. An upgrade with multiple modules in one system is possible.

///  Gas Modules

  • O2 up regulating module for the up-regulation of oxygen up to 35 %
  • O2 down regulating module for the down-regulation of oxygen down to 2 %
  • CO2 up regulating module for fermentation with a CO2-controlled gas atmosphere
  • Anaerobic cultivation set for cultivating under anaerobic conditions.

///  Fluorescence Modules

  • LED / filter module for fluorescence measurements for wavelengths between 365 nm - 950 nm
  • Low pH filter module for low pH measurements in the range of 4-6.

Up to three additional fluorescence modules can be added; in total, six different ones fit into the system.


Automated Fermentation

For fully-automated high-throughput cultivations, the BioLector will be combined with a liquid handling robotic system. Learn more about the RoboLector

  • Cell line and strain screening
  • Media screening and optimization
  • Fermentation parameter optimization
  • Anaerobic and microaerophilic fermentations
  • Synthetic and systems biology
  • Statistical design of experiments (DoE)
  • Growth characterization
  • Protein-building kinetics
  • High-throughput protein expression
  • Enzyme and cell activity tests
  • Functional genomics
  • Proteomic studies
  • Inhibition and toxicity tests
  • Quality control 

→ Please see here our application and technical notes

  • Real-time kinetics of 48 parallel fermentations
  • Microfermentation in standard MTP format
  • Combined with a robotic liquid handling module:
    •  Batch and fed-batch
    •  Media preparation
  • High-throughput and easy automation
  • Broad range for biomass detection (equivalent to up to 250 OD600, 50 g/L CDW, measured with E. coli)
  • Biomass measurement is online and does not require dilution
  • Low pH measurements in the range of 4-6
  • Small volume (800-2400 μL)
  • No edge effects
  • Continuous shaking operation (no artifacts)
  • Defined mass transfer conditions
  • Reliable scale up to benchtop fermenters
  • Industry leading data analysis software
  • Fast and easy data analysis included
  • A valuable tool for PAT and QbD 
  • System upgradable to BioLector Pro with microfluidic plate
Online Parameters
  • Biomass concentration
  • pH value
  • Dissolved oxygen (DO)
  • NAD(P)H and riboflavins
  • Fluorescent molecules (GFP, YFP, DsRed...)

System Performance

  • Temperature control (5 °C below room temperature) with active cooling
  • Humidity control (> 75 % rH)
  • Controlled gas atmosphere (CO2, O2, and N2)
Cultured Cells in the BioLector (extract)


  • Bacillus subtilis
  • Candida spec.
  • Clostridium diolis and acetobutylicum
  • Corynebacterium glutamicum
  • Escherichia coli
  • Hansenula polymorpha
  • Lactobacillus spec.
  • Paracoccus spec.
  • Penicillium spec.
  • Pichia pastoris
  • Photorhabtus luminescens
  • Saccharomyces cerevisiae
  • Schizosaccharomyces pombe
  • Streptomyces spec.
  • Tetrahymena spec.
  • Vibrio natregiens spec.
  • Yarrowia lipolytica
  • Zymomonas spec.

 Plant cells

  • Nicotiana tabacum

Cell cultures

  • CAP
  • PerC6
  • Sf9 and Sf21