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High-throughput bioprocessing that’s fast, easy and fits in any lab.

All The Best Features of The BioLector Pro – AND MO2RE.

Building on trusted BioLector Pro technology, the BioLector XT microbioreactor is based on a standard ANSI/SLAS (SBS) microtiter plate (MTP) format, and operates with online, pre-calibrated optical sensors. Disposable 48 well MTPs enable online measurement of biomass, fluorescences, pH and DO, while patented microfluidic technology supports simultaneous pH control and feeding. The optional microfluidic module eliminates manual liquid handling—no tubing/ pipetting required, as everything is part of the gamma-radiated ready-to-use plate.

New Features

Consider the benefits of these additional new features:

  • Capability to fully customize cultivation protocols to cover a broader spectrum of applications
  • Enables free combination of different feeding and pH control strategies over one cultivation run
  • Updated BioLection software provides an intuitive user interface designed for multi-user environments
  • New gassing lid reduces gas consumption and acts as an air-tight anaerobic chamber that can be used with the microfluidic module, eliminating need for anaerobic tents
  • Actively regulated O2 or CO2 concentration of ingoing gas can be raised to ≤ 100 % or ≤ 12 %, respectively


Innovative new gassing lid

  • Enables fed-batch experiments under anaerobic conditions
  • Air-tight construction supports strictly anaerobic cultivation without the need to place the entire BioLector XT microbioreactor into an anaerobic chamber
  • Gassing with O2 within a range of 1 % to 100 %
  • Gassing with CO2 within a range of 0 % to 12 %
  • Reduces gas consumption to a few mL/minute
  • Optional humidification of gases reduces evaporation

MO2RE flexibility for more applications

  • Feeding strategy development
  • Feeding rate optimization
  • Media screening and optimization
  • Cultivation parameter optimization
  • pH profiling • Strictly anaerobic and microaerophilic cultivations in batch and fed-batch mode
  • High-oxygen (up to 100 %) and high-carbon dioxide (up to 12 %) cultivations
  • Cell line and strain screening
  • Synthetic and systems biology
  • Statistical design of experiments (DoE)
  • Growth characterization
  • High-throughput protein expression
  • Enzyme and cell activity tests
  • Functional genomics
  • Proteomic studies
  • Inhibition and toxicity tests
  • Quality control

→ Please see here our application and technical notes

System Performance
  • 32/48 parallel microreactions, optionally 16 reservoir wells
  • Working volume: 800 – 2400 μL
  • Pre-calibrated optical sensors for online pH and DO measurement
  • Well-individual pH control and feeding
  • Fully flexible feeding options: constant, linear, exponential or signal-triggered, with any combinations possible
  • Broad range of kLa values (30 – 600 h-1)
  • Continuous gas exchange and oxygen supply
  • Strictly anaerobic cultivations possible, optionally with active pH control and feeding
  • Equal power input into each reactor
  • Defined engineering parameters and scalability
  • Controlled gas atmosphere (CO2, O2)
  • Temperature control (8 °C below ambient temperature to 50 °C) with active water cooling


Automated Fermentation

For triggered sampling, harvesting or additional feeding, the BioLector XT can be combined with a liquid handling robotic system. Look at the RoboLector.

“Plug and Play” Microtiter Plate Design
  • Real-time kinetics out of 48/32 parallel cultivations
  • Fully customizable and freely combinable actively regulated feeding strategies (batch, fed-batch, bolus, continuous)*
  • Control of pH on-the-plate with precalibrated optical sensors*
  • Flexible process control of pH, shaking, temperature and gassing
  • Strictly anaerobic cultivations with feeding and pH control*
  • Cultivation of highly oxygen-demanding strains with up to 100 % O2
  • DO and signal-triggered feeding*
  • Low pH measurements and control* available in the range of 4-6
  • High-throughput and easy automation
  • Broad range for biomass detection (equivalent to up to 250 OD600, 50 g/L CDW, measured with E. coli)
  • Online biomass measurement requires no dilution
  • Small working volume (800 – 2400 μL)
  • No edge effects
  • Continuous shaking operation (no artifacts)
  • Defined mass transfer conditions
  • Reliable scale-up to bench-top fermenters
  • A valuable tool for PAT and QbD

*Note: functionality requires the optional microfluidic module

Online Measurement / Online Control

MO2RE control & more data for deeper insights

Online Measurement

  • Biomass concentration
  • pH value
  • Dissolved oxygen (DO)
  • NAD(P)H and Riboflavins
  • Fluorescent molecules (e.g., GFP, YFP, DsRed)
  • Shaking speed
  • Temperature
  • O2 in head space atmosphere
  • CO2 in head space atmosphere

Online Control

  • pH value (well-specific)
  • Feeding (well-specific)
  • Shaking speed
  • Temperature
  • Gas flow
  • O2 in head space atmosphere
  • CO2 in head space atmosphere
Intelligent Software
  • Fully revised BioLection software with intuitive user interface to help support multi-user environments
  • Free programming of all control parameters
  • Open system enables live data downloads
  • Fast processor ensures rapid download of experiment data
  • Simple up- and download of protocol files and results through an intuitive protocol manager and data transfer
  • Integrated Lua scripting (optional) complements UI-supported protocol creation functionality and provides deeper access to cultivation protocol programming
Microfluidic Module

Optional Microfluidic Module helps BioLector XT microbioreactor do even MO2RE

  • Unleashes the full potential of the BioLector XT microbioreactor
  • Complements online monitoring function with well-specific pH regulation and feeding
  • Enables use of 2 reservoir wells per 4 cultivation wells—with either 2 pH-adjusting solutions, 2 feed solutions or 1 of each
  • Liquids allotted in nanoliter-scale through microvalves