2021 Publications

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Roman Jansen, Holger Morschett, Dennis Hasenklever, Matthias Moch, Wolfgang Wiechert and Marco Oldiges
Microbioreactor-assisted cultivation workflows for time-efficient phenotyping of protein producing Aspergillus niger in batch and fed-batch mode
// Fed-batch // heterologous protein production // microbioreactor // microtiter plate cultivation // Microbioreactor systems, which have been well established for bacterial organisms, enable an increased cultivation throughput via parallelization and miniaturization, as well as enhanced process insight via non-invasive online monitoring. In this study a novel workflow for Aspergillus niger was developed, allowing for up to 48 parallel microbioreactor cultivations in batch as well as fed-batch mode.
Biotechnol Progress. 2021;e3144 DOI: 10.1002/btpr.3144
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Roman Jansen, Kira Küsters, Holger Morschett, Wolfgang Wiechert and Marco Oldiges
A fully automated pipeline for the dynamic at‐line morphology analysis of microscale Aspergillus cultivation
// Aspergillus carbonarius // Morphology analysis // Microtiter plate cultivation // Microbioreactor // Projected biomass area // In this study, a protocol allowing for 48 parallel microbioreactor cultivations of Aspergillus carbonarius with non-invasive online signals of backscatter and dissolved oxygen was established. A novel and robust workflow starting from microbioreactor cultivation, automated sample harvest and processing via liquid handling robots up to automated morphology analysis was developed.
Fungal Biol Biotechnol (2021) 8:2 doi.org/10.1186/s40694-021-00109-4
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Mathias Fink, Monika Cserjan‑Puschmann, Daniela Reinisch and Gerald Striedner
High‑throughput microbioreactor provides a capable tool for early stage bioprocess development
// Fed-batch mode // bacterial production clones // high-throughput (HTP) screening // In this study, the BioLector micro-bioreactor (μ-bioreactor) system is selected as an HTP cultivation platform to screen E. coli expression clones producing representative protein candidates for biopharmaceutical applications. The outcome demonstrates the explanatory power of HTP μ-bioreactor data and the suitability of this platform as a screening tool in upstream development of microbial systems.
Scientific Reports | (2021) 11:2056 | doi.org/10.1038/s41598-021-81633-6
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Cansu Birgen, Kristin F. Degnes, Sidsel Markussen, Alexander Wentzel and Håvard Sletta
Butanol production from lignocellulosic sugars by Clostridium beijerinckii in microbioreactors
// Biofuel // Microbioreactor // Fermentation // Lignocellulosic sugar // Butanol // Clostridium // ANOVA // Renewable chemicals and fuels have gained interest worldwide as a result of increasing global warming and climate change concerns, volatility of oil price and supply as well as legal restrictions on nonrenewable energy sources. The statistical approach to evaluate the microbioreactor setup, and information obtained in this study will support further research in bioreactor and bioprocess design, which are very important aspects of industrial fermentations of lignocellulosic biomass.
Birgen et al. Biotechnol Biofuels (2021) 14:34
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