2011 Publications

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Wittgens A., Tiso T., Arndt T.T., Wenk P., Hemmerich J., Müller C., Wichmann R., Küpper B., Zwick M., Wilhelm S., Hausmann R., Syldatk C., Rosenau F. and Blank L.M.
Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440
The metabolic engineering of a rhamnolipid producing Pseudomonas putida KT2440 is reported. The metabolic network was optimized in silico for rhamnolipid synthesis and the removal of polyhydroxyalkanoate formation as competing pathway was implemented.
Microbial Cell Factories 2011, 10: 80, doi:10.1186/1475-2859-10-80
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Hemmerich J., Wenk P., Lütkepohl T. and Kensy F.
Fed-batch Cultivation in Baffled Shake-Flasks:Option Enables the Assessment of Bioprocesses in Early Development
Functional enzyme expression in E. coli using FIT fed-batch media. The combination of FIT fed-batch media and baffled shake flasks allows fed-batch fermentations with different linear feeding rates without running into strong oxygen limitations.
GEN Tutorial: Aug. 1, 2011 (Vol. 31, No. 14)
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Kensy F., Born O., Otte B. and Jennewein S.
Anaerobic High-Throughput Fermentation:New Microscale Technology with Online Monitoring Facilitates Processes for Biofuel Applications
The adaptation of m2p-labs’ BioLector® technology for anaerobic fermentations reduces dramatically complexity and effort of anaerobic fermentations by replacing experiments in shake flasks and stirred tank bioreactors.
GEN Tutorial: Feb. 15, 2011 (Vol. 31, No. 4)
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Huber R., Roth S., Rahmen N. and Büchs J.
Utilizing high-throughput experimentation to enhance specific productivity of an E. coli T7 expression system by phosphate limitation.
Investigation of the effect of different induction times and phosphate limitation on the specific productivity of the T7 expression system E.coli BL21(DE3) pRhotHi-2-EcFbFP producing EcFbFP upon induction.
BMC Biotechnology 2011, 11: 22, doi:10.1186/1472-6750-11-22
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