2013 Publications

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Matthias Ebert, Sebastian Laaß, Melanie Burghartz, Jörn Petersen,Sebastian Koßmehl, Lars Wöhlbrand, Ralf Rabus, Christoph Wittmann, Petra Tielen, Dieter Jahna
Transposon Mutagenesis Identified Chromosomal and Plasmid Genes Essential for Adaptation of the Marine Bacterium Dinoroseobacter shibae to Anaerobic Conditions
// anaerobic growth // dinoroseobacter shibae // transposon mutagenesis // Anaerobic growth and survival are integral parts of the life cycle of many marine bacteria. To identify genes essential for the anoxic life of Dinoroseobacter shibae, a transposon library was screened for strains impaired in anaerobic denitrifying growth. A novel type of anaerobic adaptation strategy was deduced
Journal of Bacteriology 2013, 195:20 p. 4769–4777, doi.org/10.1128/JB.00860-13
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Gorochowski T.E., Roubos J.A. and Bovenberg R.A.L.
Exploring Optimal Expression Using Synthetic Biology
A library of 36 strains that varied both transcriptional and translational aspects of GFP Expression was constructed, tested in a micro-reactor and monitored during expression to capture biomass and fluorescence over time.
Poster Presentation, SB6.0 Meeting, London, UK, July 9-11, 2013
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van Ooyen J., Noack S., Bott M. and Eggeling L.
Proline addition increases the efficiency of l-lysine production by Corynebacterium glutamicum
Strains with reduced citrate synthase activity were further investigated. Proline supplementation partly reversed the growth rate reduction, and simultaneously increased l-lysine accumulation leading to an increase in l-lysine productivity.
Engineering in Life Sciences, July 04, 2013, 13(4): 393–398, doi:10.1002/elsc.201200187
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Sou S. , Polizzi K. and Kontoravdi C.
Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells.
Three transfection reagents, Lipofectamine® 2000, TransIT-PRO® and linear 25 kDa polyethylenimine were evaluated for transient expression of enhanced GFP in CHO cells.
Advances in Bioscience and Biotechnology 2013, 4 (12): 1013-1019, doi:10.4236/abb.2013.412135
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Baumgart M., Luder K., Grover S., Gätgens C., Besra G.S. and Frunzke J.
IpsA, a novel LacI-type regulator, is required for inositol-derived lipid formation in Corynebacteria and Mycobacteria
Characterization of IpsA, a LacI-type transcriptional regulator conserved among Mycobacteria and Corynebacteria. Myo-inositol was identified as an effector of IpsA, causing the dissociation of the IpsA-DNA complex in vitro.
BMC Biology 2013,11: 122, doi:10.1186/1741-7007-11-122
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Klaffl S., Brocker M., Kalinowski J., Eikmanns B.J. and Bott M.
Complex Regulation of the Phosphoenolpyruvate Carboxykinase Gene pck and Characterization of Its GntR-Type Regulator IolR as a Repressor of myo-Inositol Utilization Genes in Corynebacterium glutamicum
IolR binding sites were identified in the promoter regions of iolC, iolT1 and iolR. A consensus DNA-binding motif was identified (5’ -KGWCHTRACA-3’ ) which corresponds well to those of other GntR-type regulators of the HutC family.
Journal of Bacteriology 2013, 195 (18): 4283-4296, doi:10.1128/JB.00265-13
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Becker J., Schäfer R., Kohlstedt M., Harder B.J., Borchert N.S., Stöveken N., Bremer E. and Wittmann C.
Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine.
A C. glutamicum strain was used as a cellular chassis to establish a microbial cell factory for the production of ectoines. The genome of the basic C. glutamicum strain was further engineered.
Microbial Cell Factories 2013, 12: 110, doi:10.1186/1475-2859-12-110
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Hoffmann K., Grünberger A., Lausberg F., Bott M. and Eggeling L.
Visualization of Imbalances in Sulfur Assimilation and Synthesis of Sulfur-Containing Amino Acids at the Single-Cell Level
Genetically encoded sensors which transmit elevated cytosolic concentrations of O-acetyl serine (OAS) and O-acetyl homoserine were described into an optical output.
Applied and Enviromental Microbiology 2013, 79 (21): 6730-6736, doi:10.1128/AEM.01804-13
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Baumgart M., Unthan S., Rückert C., Sivalingam J., Grünbergerr A., Kalinowski J., Bott M., Noack S. and Frunzke J.
Construction of a Prophage-Free Variant of Corynebacterium glutamicum ATCC 13032 for Use as a Platform Strain for Basic Research and Industrial Biotechnology.
The construction of MB001, a prophage-free variant of C. glutamicum ATCC 13032 with a 6% reduced genome is presented, a novel platform strain for metabolic engineering.
Applied and Enviromental Microbiology 2013, 79 (19): 6006-6015, doi:10.1128/AEM.01634-13
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Mueller F., Moussa M., El Ghazaly M., Rohde J., Bartsch N., Parthier A. and Kensy F.
Efficient production of recombinant parathyroid hormone fragment (rPTH)1-34 in the methylotrophic yeast Hansenula polymorpha.
A robust fermentation process for the efficient production of active rPTH 1-34 based on a calnexin super-transformed H. polymorpha clone was developed.
Generics and Biosimilars Initiative Journal 2013, 2 (3): 114-122, doi:10.5639/gabij.2013.0203.035
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Hemmerich J. and Kensy F.
Automation of Microbioreactors: Operating 48 Parallel Fed-Batch Fermentations at Microscale
Current methodologies in genetics and microbiology enable researchers to influence metabolic pathways of microbial cells in many directions.
BioProcess International 2013, 11 (8): 68-76
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Jensen J.V.K. and Wendisch V.F.
Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum
A platform strain overproducing ornithine with deletions of argR and argF (ORN1) has been employed for production of derived compounds such as putrescine. The product spectrum was expanded to include L-proline.
Microbial Cell Factories 2013, 12: 63, doi:10.1186/1475-2859-12-63
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Hector R.E., Dien B.S., Cotta M.A. and Mertens J.A.
Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24
A new xylose isomerase from Prevotella ruminicola TC2-24 was identified, that has one of the highest affinities and specific activities compared to other bacterial and fungal D-xylose isomerases expressed in yeast.
Biotechnology for Biofuels 2013, 6: 84, doi: 10.1186/1754-6834-6-84
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Binder S., Siedler S., Marienhagen J., Bott M. and Eggeling L.
Recombineering in Corynebacterium glutamicum combined with optical nanosensors: a general strategy for fast producer strain generation
The recombineering for C. glutamicum using RecT of prophage Rac was established and combined with nanosensor technology to enable the detection and isolation of productive mutants at the single-cell level.
Nucleic Acids Research 2013, 41 (12): 6360-6369, doi:10.1093/nar/gkt312
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Hentschel E., Will C., Mustafi N., Burkovski A., Rehm N. and Frunzke J.
Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum
SsrA-mediated peptide tagging was used for the construction of unstable variants of the GFP derivative eYFP and applied those for transient gene expression analysis in C. glutamicum.
Microbial Biotechnology 2013, 6: 196-201, doi:10.1111/j.1751-7915.2012.00360.x
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Grünberger A., van Ooyen J., Paczia N., Rohe P., Schiendzielorz G., Eggeling L., Wiechert W., Kohlheyer D. and Noack S.
Beyond growth rate 0.6: Corynebacterium glutamicum cultivated in highly diluted environments
The growth of C. glutamicum with glucose as substrate at different scales covering batch cultivations in the liter range down to single cell cultivations in the picoliter range were compared.
Biotechnology & Bioengineering 2013, 110 (1): 220-228, doi:10.1002/bit.24616
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