2018 Publications

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Stephanie J. Doong, Apoorv Gupta and Kristala L. J. Prather
Layered dynamic regulation for improving metabolic pathway productivity in Escherichia coli
Optimization of the engineered pathway for production of D-glucaric acid, a precursor to nylons and detergents, requires strategies that manage pathway competition with glycolysis as well as the stability and activity of the rate-controlling enzyme. Two orthogonal, autonomous, and tunable dynamic regulation strategies were layered to produce the highest reported glucaric acid titers in Escherichia coli K-12 strains. The implementation of two regulatory circuits harnesses synthetic biology tools to program and optimize cellular behavior and demonstrates the power of multiplexed dynamic control for strain optimization.
Proceedings of the National Academy of Sciences Mar 2018, 115 (12) 2964-2969; DOI: 10.1073/pnas.1716920115
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