Establishment of the bio-based production of glutarate, a carbon-5 dicarboxylic acid with recognized value for commercial plastics and other applications, using metabolically engineered Corynebacterium glutamicum.
Green Chem., 2018, 20, 4662, DOI: 10.1039/c8gc01901k Go to publication
Several years of fundamental and applied research made Microbioreactor (MBR) systems a common and valuable technology in biotechnology. With MBR systems large experimental datasets can be produced easily in comparably short times, shifting the bottleneck to the handling, processing, and interpretation of the underlying biological and process relevant information.
Biotechnol. J. 2018, 1700141, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, https://doi.org/10.1002/biot.201700141 Go to publication
Fermentation can be switched to a mode where cells would grow minimally and primarily act as catalysts to convert substrate into desired product - without the addition of expensive inducers. A common cell factory Saccharomyces cerevisiae is a Crabtree-positive yeast and is typically fermented at industrial scale under glucose-limited conditions to avoid the formation of ethanol.
Frontiers in Bioengineering and Biotechnology, Volume 6, 2018, Pages 63, ISSN=2296-4185, DOI: 10.3389/fbioe.2018.00063 Go to publication
Bio-based production of glutarate, a carbon-5 dicarboxylic acid with recognized value for commercial plastics and other applications, using metabolically engineered Corynebacterium glutamicum
Green Chem., 2018, 20, 4662; DOI: 10.1039/c8gc01901k Go to publication
Analysis of ROS formation and light-driven antimicrobial efficacy of eleven LOV-based FPs. In particular, the determination of singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested flavoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable differences in ROS selectivity and yield.
Scientific Reports: (2018) 8:15021; DOI:10.1038/s41598-018-33291-4 Go to publication
Optimization of the engineered pathway for production of D-glucaric acid, a precursor to nylons and detergents, requires strategies that manage pathway competition with glycolysis as well as the stability and activity of the rate-controlling enzyme. Two orthogonal, autonomous, and tunable dynamic regulation strategies were layered to produce the highest reported glucaric acid titers in Escherichia coli K-12 strains. The implementation of two regulatory circuits harnesses synthetic biology tools to program and optimize cellular behavior and demonstrates the power of multiplexed dynamic control for strain optimization.
Proceedings of the National Academy of Sciences Mar 2018, 115 (12) 2964-2969; DOI: 10.1073/pnas.1716920115 Go to publication
This work explores using high-throughput methods, real-time data capture, and automated analysis to enhance the student learning experience in applied college-level biochemical engineering lab classes. In the past, engineering laboratory classes were structured with a pre-lab lecture, one uniform assigned experiment, and data analysis done at a later date and then reported back.
Event: INTED2018; This paper is indexed in IATED Digital Library; Session: LEARNING AND TEACHING METHODOLOGIES; Session type: VIRTUAL Go to publication