2019 Publications

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Jing Shen, Jun Chen, Peter Ruhdal Jensen and Christian Solem
Development of a novel, robust and cost-efficient process for valorizing dairy waste exemplified by ethanol production
// Corynebacterium glutamicum //Lactose-utilization // Medium optimization // Ethanol production // Here we present a novel microbial platform specifically designed to generate useful compounds from dairy waste. An efficient process for producing ethanol from DWP, based on C. glutamicum, was demonstrated. The results obtained clearly show a great potential for this newly developed platform for producing value-added chemicals from dairy waste.
Microb Cell Fact. 2019; 18. doi: 10.1186/s12934-019-1091-3
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Cansu Birgen, Peter Dürre, Heinz A. Preisig and Alexander Wentzel
Butanol production from lignocellulosic biomass: revisiting fermentation performance indicators with exploratory data analysis
// Butanol // Clostridia // ABE fermentation // Lignocellulosic biomass // Mixed sugars // Exploratory data analysis // This approach provides different insights and information as a guide to a more successful fermentation of lignocellulosic sugars to butanol as a key to more competitive butanol production processes for biofuel applications in the future.
Biotechnol Biofuels. 2019; 12 doi: 10.1186/s13068-019-1508-6
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Johannes Hemmerich, Niklas Tenhaef, Carmen Steffens, Jannick Kappelmann, Marc Weiske, Sebastian J Reich, Wolfgang Wiechert, Marco Oldiges, Stephan Noack
Less Sacrifice, More Insight: Repeated Low-Volume Sampling of Microbioreactor Cultivations Enables Accelerated Deep Phenotyping of Microbial Strain Libraries
// Corynebacterium glutamicum // Mini Pilot Plant // genome reduction // heterologous protein secretion // quantitative microbial phenotyping // In this study, a novel workflow for repeated low-volume sampling of BioLector-based cultivation setups is presented.
Biotechnol J. 2019 Sep;14(9):e1800428. doi: 10.1002/biot.201800428.
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Tobias Habicher, Vroni Czotscher, Tobias Klein, Andreas Daub, Timm Keil, Jochen Büchs
Glucose-containing polymer rings enable fed-batch operation in microtiter plates with parallel online measurement of scattered light, fluorescence, dissolved oxygen tension, and pH
// BioLector // E. coli // fed-batch // high-throughput // online monitoring // In this study, a glucose-containing polymer matrix was used to manufacture polymer rings that were placed at the bottom of a 48-well microtiter plate. Thereby, the liquid content of the well became accessible for optical measurement by the BioLector device.
Biotechnol Bioeng. 2019 Sep;116(9):2250-2262. doi: 10.1002/bit.27077.
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Julia Otten, Niklas Tenhaef, Roman P Jansen, Johannes Döbber, Lisa Jungbluth, Stephan Noack, Marco Oldiges, Wolfgang Wiechert, Martina Pohl
A FRET-based biosensor for the quantification of glucose in culture supernatants of mL scale microbial cultivations
// Glucose binding protein // Micro cultivation // Online glucose measurement // Sensor beads // Venus // mTurquoise2 // A FRET-based glucose sensor was used to quantify D-glucose consumption in microtiter plate based cultivations. This is the first method reported for online quantification of D-glucose in microtiter plate based cultivations.
Microb Cell Fact. 2019 Aug 21;18(1):143. doi: 10.1186/s12934-019-1193-y.
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Fernando Pérez-García, Luciana F. Brito, and Volker F. Wendisch
Function of L-Pipecolic Acid as Compatible Solute in Corynebacterium glutamicum as Basis for Its Production Under Hyperosmolar Conditions
The effect of L-PA on C. glutamicum (either added to the culture medium or synthesized de novo) under different conditions of osmotic pressure by physiological and RNAseq experiments is characterized. Evidence that YggB may be involved in export of L-PA and ProP in its import into the C. glutamicum cell is provided.
Front Microbiol. 2019; 10: 340; Published online 2019 Feb 25. doi: 10.3389/fmicb.2019.00340
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T. Keil, B. Dittrich, C. Lattermann, T. Habicher, and J. Büchs
The two model organisms E. coli and H. polymorpha were cultivated in the fed-batch microtiter plate to investigate the general applicability for microbial systems.
The polymer-based fed-batch microtiter plate represents a sophisticated and cost efficient system to mimic typical industrial fed-batch conditions in small-scale. Thus, a more reliable strain screening and early process development can be performed. A systematical scale-down with low expenditure of work, time and money is possible.
J Biol Eng. 2019; 13: 18; Published online 2019 Feb 22. doi: 10.1186/s13036-019-0147-6
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Christian Brüsseler, Anja Späth, Sascha Sokolowsky, and Jan Marienhagen
Alone at last! – Heterologous expression of a single gene is sufficient for establishing the five-step Weimberg pathway in Corynebacterium glutamicum
The analysis of the C. glutamicum genome in combination with systematic reduction of the heterologous xylXABCD operon revealed that the hitherto unknown and endogenous dehydrogenase KsaD (Cg0535) can also oxidize α-ketoglutarate semialdehyde to the tricarboxylic acid cycle intermediate α-ketoglutarate, the final enzymatic step of the Weimberg pathway.
Metab Eng Commun. 2019 Dec; 9: e00090; Published online 2019 Apr 10. doi: 10.1016/j.mec.2019.e00090
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Holger Morschett, Roman Jansen, Christian Neuendorf, Matthias Moch, Wolfgang Wiechert, Marco Oldiges
Parallelized microscale fed-batch cultivation in online-monitored microtiter plates: implications of media composition and feed strategies for process design and performance
A workfow for 32-fold parallelized microscale cultivation of protein secreting Corynebacterium glutamicum in microtiter plates incorporating online monitoring, pH control and feeding was developed and validated.
Journal of Industrial Microbiology & Biotechnology (2019), https://doi.org/10.1007/s10295-019-02243-w
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Roman P. Jansen, Carina Beuck, Matthias Moch, Bianca Klein, Kira Küsters, Holger Morschett, Wolfgang Wiechert and Marco Oldiges
A closer look at Aspergillus: online monitoring via scattered light enables reproducible phenotyping
A robust workfow for A. giganteus was developed, allowing for reproducible microscale cultivation withonline monitoring, where calcium chloride is an useful alternative to microparticle enhanced cultivation in order tocontrol the morphology. Independent of the cultivation volume, comparable phenotypes were observed in microtiterplates and in lab-scale bioreactor.
Fungal Biology and Biotechnology, Volume 6, Article number: 11 (2019)
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Roxane Raulo, Egon Heuson, Ali Siah, Vincent Phalip and Renato Froidevaux
Innovative microscale workflow from fungi cultures to Cell Wall-Degrading Enzyme screening
Cultivations were done directly using shredded wheat straw as raw substrate in a submerged manner, proving the ability of the equipment to run accurate cultivations on such complex material with some advantages compared to the current flask cultures method (i.e. more conditions screened, less amount of substrate needed, better activities).
Microbial Biotechnology, April 2019; DOI: 10.1111/1751-7915.13405
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Maren Wehrs, Deepti Tanjore, Thomas Eng, Jeff Lievense, Todd R. Pray, and Aindrila Mukhopadhyay
Engineering Robust Production Microbes for Large-Scale Cultivation
Strain engineering in the laboratory often does not consider process requirements in larger-scale bioreactors. Systems and synthetic biology can be applied to design microbial strains that allow reliable and robust production on a large scale. Commercial microbial platforms should be selected and developed based on their relevance to final process goals.
Trends in Microbiology, 2019 (TIMI 1664 No. of Pages 14), DOI: https://doi.org/10.1016/j.tim.2019.01.006
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J. T. Boock, A. J. E. Freedman, G. A. Tompsett, S. K. Muse, A. J. Allen, L. A. Jackson, B. Castro-Dominguez, M. T. Timko, K. L. J. Prather & J. R. Thompson
Engineered microbial biofuel production and recovery under supercritical carbon dioxide
Culture contamination, end-product toxicity, and energy efficient product recovery are long-standing bioprocess challenges. The solution - a high-pressure fermentation strategy, coupled with in situ extraction using the abundant and renewable solvent supercritical carbon dioxide (scCO2), which is also known for its broad microbial lethality.
Nature Communications (2019) 10:587, https://doi.org/10.1038/s41467-019-08486-6
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