A workfow for 32-fold parallelized microscale cultivation of protein secreting Corynebacterium glutamicum in microtiter plates incorporating online monitoring, pH control and feeding was developed and validated.
Journal of Industrial Microbiology & Biotechnology (2019), https://doi.org/10.1007/s10295-019-02243-w Go to publication
A robust workfow for A. giganteus was developed, allowing for reproducible microscale cultivation withonline monitoring, where calcium chloride is an useful alternative to microparticle enhanced cultivation in order tocontrol the morphology. Independent of the cultivation volume, comparable phenotypes were observed in microtiterplates and in lab-scale bioreactor.
Fungal Biology and Biotechnology, Volume 6, Article number: 11 (2019) Go to publication
Cultivations were done directly using shredded wheat straw as raw substrate in a submerged manner, proving the ability of the equipment to run accurate cultivations on such complex material with some advantages compared to the current flask cultures method (i.e. more conditions screened, less amount of substrate needed, better activities).
Microbial Biotechnology, April 2019; DOI: 10.1111/1751-7915.13405 Go to publication
Strain engineering in the laboratory often does not consider process requirements in larger-scale bioreactors. Systems and synthetic biology can be applied to design microbial strains that allow reliable and robust production on a large scale. Commercial microbial platforms should be selected and developed based on their relevance to final process goals.
Trends in Microbiology, 2019 (TIMI 1664 No. of Pages 14), DOI: https://doi.org/10.1016/j.tim.2019.01.006 Go to publication
Culture contamination, end-product toxicity, and energy efficient product recovery are long-standing bioprocess challenges. The solution - a high-pressure fermentation strategy, coupled with in situ extraction using the abundant and renewable solvent supercritical carbon dioxide (scCO2), which is also known for its broad microbial lethality.
Nature Communications (2019) 10:587, https://doi.org/10.1038/s41467-019-08486-6 Go to publication