// Sporosarcina pasteurii // microplate cultivations // online biomass monitoring // Microbial induced calcite precipitation (MICP) based on ureolysis has a high potential for many applications, e.g. restoration of construction materials. The here proposed method to supplement media strongly improves media cost effectiveness and make MICP economically more feasible for industrial applications e.g. in the construction industry.
Scientific Reports | (2020) 10:22448 | https://doi.org/10.1038/s41598-020-79904-9 Go to publication
// machine learning // metabolic engineering // synthetic biology // deep learning // Machine learning provides researchers a unique opportunity to make metabolic engineering more predictable. In this review, an introduction to this discipline is offered in terms that are relatable to metabolic engineers, as well as providing in-depth illustrative examples leveraging omics data and improving production. A variety of applications ranging from pathway construction and optimization, to genetic editing optimization, cell factory testing, and production scale-up are discussed.
Metabolic Engineering https://doi.org/10.1016/j.ymben.2020.10.005 Go to publication
// S. pombe // cell growth // transcription factor // RNA polymerase // Cells lacking the GATA transcription factor Gaf1 grow normally after blocking TORC1. Gaf1 is required for the normal chronological lifespan of non-dividing cells. Gaf1 regulates transcription of protein-coding genes and of tRNA genes. Upon TORC1 block, Gaf1 represses tRNAs to inhibit translation and promote longevity. Thus, Gaf1 controls the transcription of both protein-coding and tRNA genes to inhibit translation and growth downstream of TORC1.
Cell Reports 30, 3240–3249, March 10, 2020 https://doi.org/10.1016/j.celrep.2020.02.058 Go to publication
// strain evaluation // wheat straw hydrolysate // In this paper, a methodology for high-throughput analysis of yeast strains directly in lignocellulosic hydrolysates is introduced. The Biolector platform was used to assess aerobic and anaerobic growth of 12 Saccharomyces cerevisiae strains and their ΔPdr12 mutants in wheat straw hydrolysate. This study demonstrates that growth in lignocellulosic hydrolysates could be rapidly monitored using 1 ml cultures and that measuring growth and product formation under relevant conditions are crucial for evaluating strain performance.
Bioresource Technology Reports 11 (2020) 100532 https://doi.org/10.1016/j.biteb.2020.100532 Go to publication
// microbial lignin conversion // Adaptive laboratory evolution // Pseudomonas putida KT2440 // Pseudomonas putida KT2440 is a promising bacterial chassis for the conversion of lignin-derived aromatic compound mixtures to biofuels and bioproducts. This work demonstrates increased tolerance towards and growth rate at the expense of hydroxycinnamic acids and presents new targets for improving P. putida for microbial lignin valorization.
Metabolic Engineering Communications 11 (2020) e00143 https://doi.org/10.1016/j.mec.2020.e00143 Go to publication
// bacterial aggregates // hydroxyectoine // encapsulation // Despite the benefits of bacterial endophytes, recent studies on the mostly Gram-negative bacteria lack of regard for formulation strategies. This research encourages the integration of fermentation and formulation strategies in a bioprocess engineering approach for exploiting endophytic bacteria.
Biotechnology Reports 26 (2020) e00463 https://doi.org/10.1016/j.btre.2020.e00463 Go to publication
// phage isolation // phage genomics // Streptomyces // Streptomyces, like all bacteria, are confronted with the constantthreat of phage predation, which in turn shapes bacterial evolution. This study expands the number of characterized phages of Streptomyces and sheds light on phage evolution and phage-host dynamics in Streptomyces.
Viruses 2020, 12(10), 1065; https://doi.org/10.3390/v12101065 Go to publication
// Transcriptional biosensor // Heterologous sensor activity // A biosensor design is introduced, which allows fine-tuning of important sensor parameters and restores the sensor response in a heterologous expression host. In this context, biosensors are constructed responding to basic amino acids or ring-hydroxylated phenylpropanoids for applications in Corynebacterium glutamicum and Escherichia coli.
Metabolic Engineering Communications 11 (2020) e00150. https://doi.org/10.1016/j.mec.2020.e00150 Go to publication
// strain adaptive laboratory evolution (ALE) // metabolic engineering // high-throughput strain screening // This multidimensional engineering of the yeast at the single protein/enzyme level, the pathway level and the cellular level, combined with an optimized cultivation process, resulted in the production of >1 g l−1 extracellular MCFAs—a more than 250-fold improvement over the original strain.
Nature Catalys is | VOL 3 | January 64 2020 | 64–74 | www.nature.com/natcatal Go to publication