When using conventional methods for media optimization, measurements are taken every couple of hours, as is limited by sampling. If an inflection is observed in a curve, the typical assumption made is that there has been an operator error in dilution or measurement. However, with the BioLector, measurements are taken so frequently (every 20 min at the most) that inflections in a measurement curve can be trusted to be a change in metabolism, such as diauxic shift or nutrient limitation. Due to the high-throughput nature of the BioLector, it is suitable for the design of experiments (DoE). Not only many nutrient components can be examined individually, but their interactions can also be examined, in order to optimize the most productive and cost-efficient media.