The anaerobic module for the BioLector system consists of an anaerobic chamber for 48-well plates and an external mass flow controller for nitrogen (N2). Alternatively, other individual gasses or gas mixtures can be supplied. Those must be non-toxic, non-flammable and non-explosive.
The MTP is filled and sealed with a foil inside an anaerobic bench. Afterwards, the anaerobic chamber is placed over the plate and sealed tightly with the screws on top of the lid. The plate-chamber combination is now protected from the outer environment and can be carried to the BioLector, maintaining the anaerobic conditions inside.
After placing the plate-chamber combination inside the BioLector, both are interconnected with the gas tubing. During fermentation, a continuous flow of N2 (or the individual gas) through the chamber is maintained. Therefore, any exposure to oxygen is prevented, providing a constant anaerobic environment.
Independent of the additional module all standard features of the microbioreactor remain unchanged.
E-AN-200 for the anaerobic module
Other Optional Modules
The combination of multiple modules is possible.
/// Gas Modules
- O2 up regulating module for the up-regulation of oxygen up to 35 %
- O2 down regulating module for the down-regulation of oxygen down to 2 %
- CO2 up regulating module for fermentation with a CO2 controlled gas atmosphere
/// Fluorescence Modules
- LED / filter module for fluorescence measurements for wavelengths between 365 nm - 800 nm
Up to three additional fluorescence modules can be added to the standard filters for biomass, pH, and DO. In total, six different modules fit into the system.